Applications
- Breath analysis
- Illness diagnosis (including pulmonary and cancer)
- Pathology
- Pharmacokinetics
- Metabolism
Features and benefits
- Direct, real-time analysis
- Up to 1000 compounds
- Outstanding operational range: 0.01-2 lpm
- Sensitivity: sub-pptv
- Liable for MS/MS or high resolution mass spectrometry
- Automated
- Soft ionization method (no damage to the molecule)
The LFSESI is an Add-On Ionizer that turns your MS into a powerful vapor analyzer for health applications
There is growing evidence that physiological processes (either pathogenic or not) have a characteristic vapor fingerprint, which can be assessed either by breath analysis or using culture media. Vapour analysis offers therefore a new universe of possibility for health diagnosis, physiological or pathological studies and pharmacokinetichs.
The new SEADM’s LFSESI (Low Flow Secondary Electrospray Ionizer) allows you to tackle these applications with unprecedented efficiency. Working as a vapor ion source in substitution of the source of your native MS, LFSESI can be seemingly assembled with your current MS (it weighs 5-6 kg depending on MS model) in less than 5 minutes to deliver up to 1000 compounds in real time, with outstanding sub-ppt sensitivities.
Benchmark
Our LFSESI has no rival in the field of vapor analysis. And still at a fraction of the price of a mass spectrometer!
| System | Mode of operation: | Number of compounds | Limit of detection (LoD) | MS/MS capability | High resolution MS capability | Automated operation |
|---|---|---|---|---|---|---|
| LFSESI-MS | Direct-Real time | 100-1000 | Sub-pptv | YES | YES | YES |
| SIFT-MS | Direct-Real time | ≈100 | ppbv | NO | NO | YES |
| PTR-MS | Direct-Real time | ≈100 | pptv | NO | NO | YES |
| GC-MS | Pre-concentration | 100-1000 | Sub-pptv | YES | YES | NO |
References: Metabolites 2014, 4, 465-498; Anal. Chem., 2016, 88 (4), pp 2406–2412.
For more publications on the potential of our LFSESI technology, please check the work of Pablo Martinez-Lozano Sinués
Application examples
Take a look at some of customer application examples and discover what LFSESI can do to boost your research!
| Area | Object of the study | Samples obtained from: | Publication (click link to access article) | Ref. |
|---|---|---|---|---|
 PHYSIOLOGY |
Circadian clocks | Human breath (3 individuals) | Circadian variation of the human metabolome captured by real-time breath analysis | PLoS ONE9(12): e114422 |
| Aminoacids in blood | Human breath (37) | Real-time quantification of amino acids in the exhalome by secondary electrospray ionization-mass spectrometry: A proof-of-principle study | Clinical Chemistry 62:9 1230–1237 (2016) | |
 PATHOLOGY |
Oral bacteria | In vitro bacteria samples | Differentiation of oral bacteria in in vitrocultures and human saliva by secondary electrospray ionization – mass spectrometry | Scientific Reports 5, Article number: 15163 (2015) |
 DISEASE DIAGNOSIS |
Pulmonary fibrosis disease | Human breath (21) | 119 Exhaled breath analysis by real-time mass spectrometry in patients with pulmonary fibrosis | Chest 2017 Vol. 151, Issue 5, Supplement, Page A16 |
| Cancer disease | Culture media | Fingerprinting breast cancer vs. normal mammary cells by mass spectrometric analysis of volatiles | Sci Rep. 2014; 4: 5196. | |
| Chronic Obstructive Pulmonary Disease | Human breath (61) | Breath analysis in real time by mass spectrometry in chronic obstructive pulmonary disease | Respiration (2014) 87(4) | |
 PHARMACOKYNETICS |
Salbutamol effects | Human breath (98) | Metabolic effects of inhaled salbutamol determined by exhaled breath analysis | J. Breath Res. 11 (2017) 046004 |
| Ketamine, propofol, valproic acid | Mice breath | Drug Pharmacokinetics Determined by Real-Time Analysis of Mouse Breath | Angew. Chem. Int. Ed. 2015, 54, 1 – 5 |